Green fluorescent protein as a reporter to monitor gene expression and food colonization by Aspergillus flavus.

نویسندگان

  • W Du
  • Z Huang
  • J E Flaherty
  • K Wells
  • G A Payne
چکیده

Transformants of Aspergillus flavus containing the Aequorea victoria gfp gene fused to a viral promoter or the promoter region and 483 bp of the coding region of A. flavus aflR expressed green fluorescence detectable without a microscope or filters. Expression of green fluorescent protein fluorescence was correlated with resistance to aflatoxin accumulation in five corn genotypes inoculated with these transformants.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 65 2  شماره 

صفحات  -

تاریخ انتشار 1999